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Institutional
Biological & Chemical Safety Committee (IBC)
Points to consider when using viral vectors
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List the genes/siRNA/shRNA
being expressed as well as a brief description of the function
(known or putative) of the targeted gene/siRNA/shRNA
-
Is expression
transient or stable?
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What is the mechanism of attenuation
of the vector?
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What is the probability of
reversion to a virulent phenotype?
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What is the potential for integration
into the host genome?
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What is the infection tropism
(human, animal, plant, etc.) of both the original source virus
and the
viral
vector?
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What are the factors which
make it more or less pathogenic than the source virus?
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